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Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Reagents and Kits (132)
Real Time PCR Master Mixes (122)
Real Time PCR Assays and Arrays (15)

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1

Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits

The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.

EDGE
Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell HEK
PCR Method qPCR
Formato Kit
2

Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits

The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).

EDGE
Quantità 100 Reactions
Label or Dye SYBR Green
Da utilizzare con (applicazione) Mycoplasma Detection
Descrizione Detection for BioProduction process media, cells, raw materials
Linea di prodotti MycoSEQ
3

TaqMan™ GMO Screening Kit

TaqMan™ GMO Screening Kit

EDGE
4

Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System

The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.

EDGE
5

Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Forma Liquid
Metodo di rilevamento Primer-probe
Product Type Mycoplasma Detection
Reaction Speed Standard
Label or Dye FAM
N. di reazioni 100 Reactions
Da utilizzare con (applicazione) Microbial Detection
Linea di prodotti MycoSEQ
Tipo di campione Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
PCR Method Multiplex qPCR
Target Organism Class Mollicutes, mycoplasma
6

Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits

The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.

EDGE
Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM + VIC
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell SF9 + Bacoluvirus
PCR Method qPCR
Formato Kit
7

Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell CHO
PCR Method qPCR
Formato Kit
8

Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits

This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell E.coli
PCR Method qPCR
Formato Kit
9

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade UP (Ultra Pure)
Polimerasi AmpliTaq Gold DNA Polymerase
Linea di prodotti TaqMan
PCR Method qPCR
Formato Bottle
Da utilizzare con (apparecchiatura) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Metodo di rilevamento Primer-probe
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Hot Start Built-In Hot Start
Da utilizzare con (applicazione) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
Tipo di campione Environmental Sample, Food
Concentrazione 2X
11

Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kits

The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell Vero
PCR Method qPCR
Formato Kit
12
Promozioni disponibili

Applied Biosystems™ TaqMan™ MGB Probe
GREENER_CHOICE

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.

Da utilizzare con (apparecchiatura) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Purity or Quality Grade HPLC
Forma Liquid
Internal Probe Modification None
Da utilizzare con (applicazione) Real Time PCR (qPCR)
Metodo di purificazione HPLC
Linea di prodotti TaqMan
3'Primer Modification MGB-NFQ
Formato Tube
13

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
Host Cell Human DNA
PCR Method qPCR
Formato Kit
14

Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits

The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.

Quantità 100 reactions
Metodo di rilevamento Primer-probe
Label or Dye FAM
N. di reazioni 100 Reactions
Linea di prodotti resDNASEQ
PCR Method qPCR
Bersaglio Kanamycin Resistant Plasmids
Formato Kit
15

Applied Biosystems™ resDNASEQ™ dPCR E. coli DNA Kit

The Applied Biosystems resDNASEQ dPCR E. coli DNA Kit is a digital PCR (dPCR)-based solution to detect host-cell DNA from E. coli, an expression system commonly used for production of recombinant proteins and plasmids.